I wanted to track convective flow in rehydrating aqueous two-phase systems to improve ELISAs, but tools to observe flow were hard to find. I saw a super-resolution microscope technique in a class at Michigan with Dr. Andy Putnam and thought, AHA! I can adapt this to track beads flowing in 3D! I checked in with Ryan Oliver, who helped me build the astigmatism into an old microscope, we wrote some software, and now we can track 3D micro-scale flow. Taisuke Kojima helped me run analysis on a few ATPS compositions, and we sent off the proof-of-concept paper. Thanks as usual to Shuichi Takayama for contributions all around.
Check out the paper here: https://www.frontiersin.org/articles/10.3389/fchem.2019.00311/full
Yamanishi Research 